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Journal Articles

Effect of heavy ion irradiation to the silkworm eggs at before fertilization and at nuclear cleavage stage

Ueda, Daisuke*; Shirai, Koji*; Funayama, Tomoo; Sakashita, Tetsuya; Yokota, Yuichiro; Kobayashi, Yasuhiko

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 84, 2015/03

In this study, we investigated the effects of irradiation to the silkworm eggs at various developmental stages. First, we tried the irradiation to the unfertilized eggs (at 1.5 hour after oviposition). At this stage, the female pronucleus and the sperm nucleus are observed in the eggs, but not fertilized. After irradiation, the irradiated eggs stopped the development after fertilization. About 2 hours after, the egg restarted the nuclear cleavage. This result indicates the DNA damage on pronuleus cannot prevent the fertilization. We also investigated the effects of irradiation to the egg at the nuclear cleavage stage (at 6 hour after oviposition). The egg also stopped the development after irradiation, but the duration time of the developmental arrest was almost two times longer (about 4 hours) than that of the egg irradiated at fertilization.

Journal Articles

Fate mapping of the cellular blastoderm stage egg of the silkworm, ${{it Bombyx mori}}$, by localized irradiation of heavy ion microbeams

Kiguchi, Kenji*; Kinjo, Y.*; Masahashi, K.*; Tu, Z.; Tamura, Hajime*; Shirai, K.*; Kanekatsu, Rensuke*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi

JAERI-Review 2000-024, TIARA Annual Report 1999, p.51 - 53, 2000/10

no abstracts in English

Journal Articles

Effects of heavy ion microbeams on embryogenesis in the silkworm, ${{it Bombyx mori}}$; Morphological changes of the nuclei and cells after spot irradiation

Kiguchi, Kenji*; Shima, T.*; Kinjo, Y.*; Tu, Z. L.*; Yamasaki, Shuhei*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi

JAERI-Review 99-025, TIARA Annual Report 1998, p.53 - 55, 1999/10

no abstracts in English

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